You are here

Epigenetic effects of microplastics exposure on the common mysid shrimp, americamysis bahia

Download pdf | Full Screen View

Date Issued:
2020
Abstract:
Microplastics are publicly recognized as a ubiquitous issue of environmental concern in aquatic systems. Primary consumers of microplastics are often zooplankton of biological and commercial importance, including the mysid shrimp, Americamysis bahia. In the presence of environmental stressors, gene expression may be altered without changing DNA sequences. This can occur through methylation of the DNA, in which a methyl group binds to nucleotide base pairs, most commonly cytosine. In this study, exposure to 5 micrometer microbeads at different concentrations caused no significant effect on mortality within 72 hours of exposure, but significantly increased mortality thereafter. Microplastics were shown to interact and accumulate on mysid bodies in various ways that would likely cause stress. An ELISA-like colorimetric assay was used to assess mysid shrimp genomic DNA for differences in global percent methylation that are potentially associated with microplastics exposure. No significant difference in the average percent of methylated DNA was found between treatment groups. There was no significant difference in the number methylation detections between treatments. This is the first study that has detected DNA methylation in any member of the order Mysida. This is the first study that has investigated DNA methylation effects as a result of microplastic induced stress.
Title: Epigenetic effects of microplastics exposure on the common mysid shrimp, americamysis bahia.
36 views
10 downloads
Name(s): Prior, Jack Hamilton, author.
Type of Resource: text
Genre: Bibliography
Text-txt
Crustacea
Microplastics
Academic Theses.
Academic Theses.
Electronic Thesis Or Dissertation.
Issuance: monographic
Date Issued: 2020
Date Issued: 2020
Other Date: 2020.
Publisher: University of West Florida,
Place of Publication: Pensacola, Florida :
Physical Form: electronic resource
Extent: 1 online resource (x, 97 leaves : illustrations, charts)
Language(s): eng
Abstract: Microplastics are publicly recognized as a ubiquitous issue of environmental concern in aquatic systems. Primary consumers of microplastics are often zooplankton of biological and commercial importance, including the mysid shrimp, Americamysis bahia. In the presence of environmental stressors, gene expression may be altered without changing DNA sequences. This can occur through methylation of the DNA, in which a methyl group binds to nucleotide base pairs, most commonly cytosine. In this study, exposure to 5 micrometer microbeads at different concentrations caused no significant effect on mortality within 72 hours of exposure, but significantly increased mortality thereafter. Microplastics were shown to interact and accumulate on mysid bodies in various ways that would likely cause stress. An ELISA-like colorimetric assay was used to assess mysid shrimp genomic DNA for differences in global percent methylation that are potentially associated with microplastics exposure. No significant difference in the average percent of methylated DNA was found between treatment groups. There was no significant difference in the number methylation detections between treatments. This is the first study that has detected DNA methylation in any member of the order Mysida. This is the first study that has investigated DNA methylation effects as a result of microplastic induced stress.
Identifier: 1206362804 (oclc), WFE0000728 (IID)
Note(s): by Jack Hamilton Prior.
Department of Biology, Hal Marcus College of Science and Engineering
Thesis (M.S.) University of West Florida 2020
Includes bibliographical references.
Also available in print.
Subject(s): University of West Florida
University of West Florida.
Crustacea
Microplastics
Library Classification: LD1807.F62k 2020 P756
Persistent Link to This Record: http://purl.flvc.org/uwf/fd/WFE0000728
Use and Reproduction: http://rightsstatements.org/vocab/InC-EDU/1.0/
Host Institution: UWF
Other Format: Epigenetic effects of microplastics exposure on the common mysid shrimp, americamysis bahia. (Print version:)
(OCoLC)1206362731

In Collections