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Title
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Oral hygiene regimes: how oil pulling and conventional mouthwashes affect dental biofilms, focus on Cariogenic Streptococci.
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Author
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Bercier, Charles H.
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Abstract/Description
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This research addresses whether nontraditional oral hygiene regimes provide antimicrobial efficacy comparable to commercially available synthetic mouthwashes. A common interest among holistic communities is "oil pulling"--a practice originating in ancient Ayurveda oral hygiene. It consists of rinsing the mouth with vegetable oils, of which sesame and coconut oils are preferred, to enhance oral hygiene. This study focused on whether oil pulling reduces cariogenic Streptococcus mutans to the...
Show moreThis research addresses whether nontraditional oral hygiene regimes provide antimicrobial efficacy comparable to commercially available synthetic mouthwashes. A common interest among holistic communities is "oil pulling"--a practice originating in ancient Ayurveda oral hygiene. It consists of rinsing the mouth with vegetable oils, of which sesame and coconut oils are preferred, to enhance oral hygiene. This study focused on whether oil pulling reduces cariogenic Streptococcus mutans to the same degree as does common mouthwash. Volunteers above age 18 were to participate in this UWF-IRB-approved study. Participants would provide oral rinses of 20mL with sterile saline before and after treatments of Listerine®, or pulling oil; the rinses were transported to the lab to test for S. mutans and other oral bacteria. After the first round of treatments were complete, a 1-week "reset" period allowed normal microbial flora to re-establish, after which another treatment regime began; the cycle continued until all participants underwent all treatments. Samples were plated on a highly selective medium, favoring growth of S. mutans. Samples were also used to inoculate two broth dilution-tube series consisting of 1) a non-selective general medium TSB, and 2) a S. mutans-selective medium. Preliminary trials of this protocol showed that Listerine® reduced S. mutans and other oral bacteria by over 1-thousand-fold. Furthermore, almost all visible background growth was eliminated from the media plates after Listerine® treatment.
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Date Issued
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2020
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Identifier
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1153936619, WFE0000704
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Format
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Document (PDF)