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Title
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Antioxidant mechanisms and bioactivity of phenolic compunds found in Dioscorea Bulbifera.
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Author
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Rees, Patricia Elizabeth
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Abstract/Description
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Accumulation of oxidative damage has been implicated in numerous diseases bringing antioxidant investigations and plant phenolics to the forefront of medicinal research. The species Dioscorea bulbifera has been used in both traditional and modern medicinal systems and has good overall antioxidant capacity. Yet little work has been done to assess which chemical components may contribute to this action. The goals of this study were to investigate the antioxidant activity of individual phenolic...
Show moreAccumulation of oxidative damage has been implicated in numerous diseases bringing antioxidant investigations and plant phenolics to the forefront of medicinal research. The species Dioscorea bulbifera has been used in both traditional and modern medicinal systems and has good overall antioxidant capacity. Yet little work has been done to assess which chemical components may contribute to this action. The goals of this study were to investigate the antioxidant activity of individual phenolic compounds in the plant D. bulbifera; to compare the in vitro antioxidant assays with human cell studies; for this information to contribute to future investigations in treating human disease. Methanolic extracts of the D. bulbifera bulbils were analyzed using HPLC-MS/MS and NMR. Positively identified compounds were used for in vitro antioxidant assays (Ferric Reducing Antioxidant Power (FRAP), iron chelation, and Oxygen Radical Absorbance Capacity (ORAC), followed by human cell assays. Several phenolic species were tentatively identified. (+)-catechin was positively identified and used for further testing. In vitro assays showed potential for electron transfer antioxidant activity but not iron chelation. The hydrogen transfer mechanisms could not be assessed due to assay complications. Cell assays also suffered complications rendering them inconclusive.
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Identifier
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1129599262, WFE0000671
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Format
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Document (PDF)